Coding

Part:BBa_M1018:Design

Designed by: Michaela Hugie   Group: UtahState_BE5930_-_S11   (2011-04-19)


PhaB with zif23-GCN4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 707
    Illegal AgeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is supposed to be a silver fusion compatible sequence. With no stop codon and a scar between the zif23-GCN4 and the PhaB sequence.


Source

N/A

References